In 1904, scientific papers were being published suggesting that bacteria were generating different acids that could be involved in the alteration and subsequent dissolution of rocks. A definitive paper was published in 1910 that demonstrated that bacteria indeed were involved in rock decay. In 1933, an additional paper was published that confirmed earlier work in this area. There results attested to the fact that of the 58 strains of isolated, viable bacteria, all fell into a category either of fermenting, sulphate reducing, and nitrifying bacteria. Many of the strains of bacteria were found to produced CO2 whereas others produced sulphuric, and nitric acid. These microbes were found not only living on the surface of the host rock but two feet deep into the stone.
Research into cave formation in modern-day carbonate platforms has generated virtually parallel results from the 1933 work done mostly in Europe. As in the 1933 research, we have identified viable bacterial populations not only in the rocks in caves currently above sea level but also in the rock of the caves currently in the phreatic zone. As in earlier work, we have shown that the bacterial population that lives in the dry cave rock is different from those that are found in the drip water. We were able to isolate 12 different species of bacteria in the dry wall rock of which two species, which, were isolated in the stones, were the same found in rainwater. CO2 measurements made in Altar Cave revealed a CO2 production rate for that time of collection of 2.2 g CO2/kg/day, (R=0.7) compared to 0.13g CO2/kg/day from stone.